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The Automatic Choice For Spectroscopy, Chromatography and Electrophoresis

PeakFit automatically places peaks in three ways.

PeakFit uses three programs to automatically place hidden peaks; While each approach is a powerful solution, one approach may work better for certain data sets than others.

The residual process initially places peaks by looking for local maxima in a smooth data stream. Then optionally add hidden peaks where they occur in the residuals.

The second derivative process searches for local minima in a smooth second derivative data stream. These local minima usually show hidden peaks.

The deconvolution process uses a Gaussian response function with Fourier deconvolution/filtering algorithm. The spectrum of a successful deconvolution will consist of "sharpened" peaks of equivalent area. The goal is to enhance the hidden peaks so that each peak represents a local maximum.




Full Graphical Placement of Peaks.

If PeakFit’s auto-placement features fail on extremely complicated or noisy data, you can place and fit peaks graphically with only a few mouse clicks. Each placed function has “anchors” that adjust even the most highly complex functions, automatically changing that function’s specific numeric parameters. PeakFit’s graphical placement options handle even the most complex peaks as smoothly as Gaussians.


PeakFit Offers Sophisticated Data Manipulation.

With PeakFit’s visual FFT filter, you can inspect your data stream in the Fourier domain and zero higher frequency points — and see your results immediately in the time-domain. This smoothing technique allows for superb noise reduction while maintaining the integrity of the original data stream.


PeakFit also includes an automated FFT method as well as Gaussian convolution, the Savitzky-Golay method and the Loess algorithm for smoothing. AI Experts throughout the smoothing options and other parts of the program automatically help you to set many adjustments. And, PeakFit even has a digital data enhancer, which helps to analyze your sparse data. Only PeakFit offers so many different methods of data manipulation.


Highly Advanced Baseline Subtraction

PeakFit’s non-parametric baseline fitting routine easily removes the complex background of a DNA electrophoresis sample. PeakFit can also subtract eight other built-in baseline equations or it can subtract any baseline you’ve developed and stored in a file.


Publication-Quality Graphs and Data Output

Every publication-quality graph (see above) was created using PeakFit’s built-in graphic engine — which now includes print preview and extensive file and clipboard export options. The numerical output is customizable so that you see only the content you want.


PeakFit Saves You Precious Research Time

For most data sets, PeakFit does all the work for you. What once took hours now takes minutes – with only a few clicks of the mouse! It’s so easy that novices can learn how to use PeakFit in no time. And if you have extremely complex or noisy data sets, the sophistication and depth of PeakFit’s data manipulation techniques is unequaled.


Product Features

The product features for PeakFit are detailed below in the following categories:

Data Input

  • Excel®
  • Lotus 123®
  • Quattro Pro®Windows®
  • SigmaPlot®
  • AIA Chromatography
  • dBase ® III+, IV
  • DIF
  • ASCII and Spreadsheet – like Editors
  • Averaging Digital Filter


Data Preparation

  • Gaussian Deconvolution to remove Spectrophotometer Instrument Response smearing
  • Exponential Deconvolution to remove Chromatographic Detector Response smearing
  • Smoothing (Savitsky Golay, FFT Filter, Loess, Gaussian Convolution)
  • Real – time FFT/Time Domain Graphical Editor
  • Dual Graph Data Sectioning with Graphical data point exclusion
  • Non – Parametric Digital Filter to Filter or augment data
  • Compare with Reference
  • Subtract Baseline Imported from File
  • Data Transforms
  • Area Normalization
  • Inspect Second and Fourth Derivatives
  • Data Weighting


Peak Autoplacement

  • Automatic by Local Maxima and Residuals
  • Automatic by Second Derivatives
  • Automatic by Deconvolved Local Maxima
  • Graphical Placement and Adjustments
  • Manual Parameter Adjustments
  • Share and Lock Parameters
  • Constant or V ariable Widths and/or Shape in a single step


Non-Linear Curve Fitting

  • Marquardt – Levenburg Algorithm
  • 83 built-in nonlinear peak models
  • Least – Squares and 3 Robust (Maximum Likelihood) methods
  • Up to 100 Peaks and 1000 Parameters
  • Intelligent Constraints to insure Fit Integrity
  • Sparse Curvature Matrix for Faster Fitting
  • Both Numeric and Graphical Fitting Options
  • Zoom – in or Toggle Points during Fitting


Output and Export Options

  • File Export with full Generated Data: Lotus 123, Excel, Quattro Pro Windows, SigmaPlot, and ASCII
  • Graphs to clipboard or file in BMP or WMF formats
  • All Numeric data in Graph to Clipboard in Spreadsheet Format


Peak Fitting

The Fitting Functions and Features for PeakFit by Systat Software are shown below:


Built-In Nonlinear Functions (83 total)

  • Spectroscopy (18): Gauss Amp, Gauss Area, Lorentz Amp, Lorentz Area, Voigt Amp, Voigt Area, Voigt Amp Approx, Voigt Amp G/L, Voigt Area G/l, Gauss Cnstr Amp, Gauss Cnstr Area, Pearson VII Amp, Pearson VII Area, Gauss+Lor Area, Gauss*Lor, Gamma Ray, Compton Edge
  • Chromatography (8): HVL, NLC, Giddings, EMG, GMG, EMG+GMG, GEMG, GEMG 5-parm
  • Statistical (31): Log Normal Amp, Log Normal Area, Logistic Amp, Logistic Area, Laplace Amp, Laplace Area, Extr Value Amp, Extr Value Area, Log Normal-4 Amp, Log Normal-4 Area, Eval4 Amp Tailed, Eval4 Area Tailed, Eval4 Amp Frtd, Eval4 Area Frtd, Gamma Amp, Gamma Area, Inv Gamma Amp, Inv Gamma Area, Weibull Amp, Weibull Area, Error Amp, Error Area, Chi-Sq Amp, Chi-Sq Area, Student t Amp, Student t Area, Beta Amp, Beta Area, F Variance Amp, F Variance Area, Pearson IV
  • General Peak (12): Erfc Pk, Pulse Pk, LDR Pk, Asym Lgstc Pk, Lgstc pow Pk, Pulse pow Pk, Pulse Wid2 Pk, Intermediate Pk, Sym Dbl Sigmoid, Sym Dbl GaussCum, Asym Dbl Sigmoid, Asym Dbl GaussCum
  • Transition (14): Sigmoid Asc, Sigmoid Desc, GaussCum Asc, GaussCum Desc, LorentzCum Asc, LorentzCum Desc, LgstcDose Rsp Asc, LgstcDoseRsp Desc, LogNormCum Asc, LogNormCum Desc, ExtrValCum Asc, ExtrValCum Desc, PulseCum Asc, PulseCum Desc


User Defined Functions

  • Up to 10 Parameters per function
  • Up to 15 UDF’s active during fit
  • Estimates can contain formulas and constraints
  • Extensive mathematical, statistical, Bessel, and logic functions


Baseline Fit and Subtract

  • Automatic detection of baseline points by constant second derivatives
  • Real-time Fitting in conjunction with data point selection, deselection
  • Background Functions (10): Constant, Linear, Progressive Linear, Quadratic, Cubic, Logarithmic, Exponential, Power, Hyperbolic and Non-Parametric


Graphical Review

  • Component and Sum Curve graphs
  • Residuals Graphs, including Distribution and Stabilized Normal Probability Plots
  • Confidence and Prediction Intervals
  • Peak Labels (Amplitude, Center, or Area)


Numerical Review

  • Peak Characterization data: Center, Amplitude, Integrated Area, Analytical Area, FWHM, FW10, FWBASE, Asymmetry at HM, Asymmetry at 10 percent, First and Second Moments, Column Efficiency and Resolution, Percentage Areas, Overlap Areas
  • Parameter Statistics: Parameter Values, Confidence Limits (90, 95, 99 percent), t-values, Standard Errors
  • Fit Statistics: Analysis of Variance, F-statistic, Overall Standard Error, r2 value
  • Data Statistics: Residual Values, Predicted Y-values, Confidence/Prediction Intervals


System Requirments

Below are the minimum requirements to run PeakFit:



  • 486 Processor or higher
  • 16 MB RAM required (32 MB or more recommended)
  • 10MB hard disk space


Windows 10, 8.x,  7, Windows Vista, 95, 98, NT and XP (32 bit)




PeakFit Is The Automatic Choice For Spectroscopy, 

Chromatography Or Electrophoresis 


Suggested uses for PeakFit are represented below however, PeakFit can be used in any science, research or engineering discipline.


For Spectroscopy

PeakFit lets you accurately detect, separate and quantify hidden peaks that standard instrumentation would miss. PeakFit includes 18 different nonlinear spectral application line shapes, including the Gaussian, the Lorentzian, and the Voigt, and even a Gaussian plus Compton Edge model for fitting Gamma Ray peaks. As a product of the curve fitting process, PeakFit reports amplitude (intensity), area, center and width data for each peak. Overall area is determined by integrating the peak equations in the entire model. PeakFit can even deconvolve your spectral instrument response so that you can analyze your data without the smearing that your instrument introduces.


For Electrophoresis

PeakFit gives the electrophoresis user the ability to quickly and easily separate, locate and measure up to 100 peaks (bands), even if they overlap. With 82 nonlinear peak models to choose from, you’re almost guaranteed to find the best equation for your data. The sophisticated array of baseline types lets you integrate only the significant portion of the bands in your data. The results of these measurements are then automatically recorded in a PeakFit-generated report, or they can be shown graphically.


For Chromatography

PeakFit includes 8 different built-in equations for asymmetric peaks typically found in chromatography data including the Exponentially-Modified Gaussian, the Haarhoff-Van der Linde, the NLC, the Giddings and even a Half-Gaussian Modified Gaussian, an experimental model similar to the EMG, but used to describe intra-column originated asymmetry. The peak area is computed directly as a parameter within each of these functions, ensuring accuracy and enabling computation of confidence limits. PeakFit reports column efficiency, resolution, first moment (center of mass), second moment, center (mode), as well as peak width at base and half maximum, and asymmetry at base and 10 percent of maximum. PeakFit can even deconvolve your chromatographic detector response, so you can analyze your data without instrument induced asymmetry. PeakFit also supports AIA data file input protocol.


For Signal Component Analysis

Even though PeakFit is widely used by chemists, biologists, etc., many Electrical Engineers have found PeakFit’s statistical nonlinear fitting techniques extremely useful for separating overlapping signals.


A True Voigt Function

In Spectroscopy, instrument and Doppler broadening effects create a Gaussian line shape, while natural and collision broadening cause a Lorentzian line shape. The Voigt function is a convolution of both the Gaussian and Lorentzian functions. Most analysis packages that offer a Voigt function use an approximation with very limited precision. PeakFit actually uses a closed-form solution to precisely calculate the function analytically. PeakFit has four different Voigt functions, so you can fit the parameters you’re most interested in, including the individual widths of both the Gaussian and Lorentzian components, and also the amplitude and area of the Voigt function. PeakFit’s precise calculation of the Voigt function is crucial to the accuracy of your analysis.


Nonlinear Peak Separation and Analysis for Spectroscopy, Chromatography and Electrophoresis

  • PeakFit separates and analyzes nonlinear peak data better, more accurately and more conveniently than your lab instrument – here’s why:
  • Advanced nonlinear curve fitting techniques separate overlapping peaks that standard instrumentation misses
  • Peak finding and fitting is fully automated! PeakFit has three different procedures to accurately characterize normal and hidden peaks
  • Graphically fit peaks yourself when you want to override PeakFit’s automation
  • Highly advanced data smoothing and manipulation techniques accurately process very noise data
  • Revolutionary constant zero second derivates base-line fitting routine finds the best baseline – PeakFit also includes a non-parametric model for extremely complex backgrounds
  • 82 built-in nonlinear equations (18 for spectroscopy and 8 for chromatography) and up to 15 user-defined functions fit virtually all data
  • Fit up to 100 peaks at a time
  • With PeakFit, your accuracy will increase, and you’ll find and fit your peaks faster than ever


Don’t guess or rely on the accuracy of non-dedicated software made by hardware manufacturers. PeakFit far surpasses any other peak analysis package made!

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