PeakFit
PeakFit Automatically Places Peaks in Three Ways
PeakFit uses three procedures to automatically place hidden peaks; while each is a strong solution, one method may work better with some data sets than others.
The Residuals procedure initially places peaks by finding local maxima in a smoothed data stream. Hidden peaks are then optionally added where peaks in the residuals occur.
The Second Derivative procedure searches for local minima within a smoothed second derivative data stream. These local minima often reveal hidden peaks.
The Deconvolution procedure uses a Gaussian response function with a Fourier deconvolution / filtering algorithm. A successfully deconvolved spectrum will consist of “sharpened” peaks of equivalent area. The goal is to enhance the hidden peaks so that each represents a local maximum.
[toggle border=’2′ title=’Full Graphical Placement of Peaks’] If PeakFit’s auto-placement features fail on extremely complicated or noisy data, you can place and fit peaks graphically with only a few mouse clicks. Each placed function has “anchors” that adjust even the most highly complex functions, automatically changing that function’s specific numeric parameters. PeakFit’s graphical placement options handle even the most complex peaks as smoothly as Gaussians.
[/toggle] [toggle border=’2′ title=’PeakFit Offers Sophisticated Data Manipulation’] With PeakFit’s visual FFT filter, you can inspect your data stream in the Fourier domain and zero higher frequency points — and see your results immediately in the time-domain. This smoothing technique allows for superb noise reduction while maintaining the integrity of the original data stream.
PeakFit also includes an automated FFT method as well as Gaussian convolution, the Savitzky-Golay method and the Loess algorithm for smoothing. AI Experts throughout the smoothing options and other parts of the program automatically help you to set many adjustments. And, PeakFit even has a digital data enhancer, which helps to analyze your sparse data. Only PeakFit offers so many different methods of data manipulation.
[/toggle] [toggle border=’2′ title=’Highly Advanced Baseline Subtraction’] PeakFit’s non-parametric baseline fitting routine easily removes the complex background of a DNA electrophoresis sample. PeakFit can also subtract eight other built-in baseline equations or it can subtract any baseline you’ve developed and stored in a file.
[/toggle] [toggle border=’2′ title=’Publication-Quality Graphs and Data Output’] Every publication-quality graph (see above) was created using PeakFit’s built-in graphic engine — which now includes print preview and extensive file and clipboard export options. The numerical output is customizable so that you see only the content you want.
[/toggle] [toggle border=’2′ title=’PeakFit Saves You Precious Research Time’] For most data sets, PeakFit does all the work for you. What once took hours now takes minutes – with only a few clicks of the mouse! It’s so easy that novices can learn how to use PeakFit in no time. And if you have extremely complex or noisy data sets, the sophistication and depth of PeakFit’s data manipulation techniques is unequaled.
The product features for PeakFit are detailed below in the following categories:
[toggle border=’2′ title=’Data Input’]
[/toggle] [toggle border=’2′ title=’Data Preparation’]
[/toggle] [toggle border=’2′ title=’Peak Autoplacement’]
[/toggle] [toggle border=’2′ title=’Non-Linear Curve Fitting’]
[/toggle] [toggle border=’2′ title=’Output and Export Options’]
The Fitting Functions and Features for PeakFit by Systat Software are shown below:
[toggle border=’2′ title=’Built-In Nonlinear Functions (83 total)’]
[/toggle] [toggle border=’2′ title=’User Defined Functions’]
[/toggle] [toggle border=’2′ title=’Baseline Fit and Subtract’]
[/toggle] [toggle border=’2′ title=’Graphical Review’]
[/toggle] [toggle border=’2′ title=’Numerical Review’]
Below are the minimum requirements to run PeakFit:
Windows 10, 8.x, 7, Windows Vista, 95, 98, NT and XP (32 bit)
Suggested uses for PeakFit are represented below however, PeakFit can be used in any science, research or engineering discipline.
[toggle border=’2′ title=’For Spectroscopy’] PeakFit lets you accurately detect, separate and quantify hidden peaks that standard instrumentation would miss. PeakFit includes 18 different nonlinear spectral application line shapes, including the Gaussian, the Lorentzian, and the Voigt, and even a Gaussian plus Compton Edge model for fitting Gamma Ray peaks. As a product of the curve fitting process, PeakFit reports amplitude (intensity), area, center and width data for each peak. Overall area is determined by integrating the peak equations in the entire model. PeakFit can even deconvolve your spectral instrument response so that you can analyze your data without the smearing that your instrument introduces.
[/toggle] [toggle border=’2′ title=’For Electrophoresis’] PeakFit gives the electrophoresis user the ability to quickly and easily separate, locate and measure up to 100 peaks (bands), even if they overlap. With 82 nonlinear peak models to choose from, you’re almost guaranteed to find the best equation for your data. The sophisticated array of baseline types lets you integrate only the significant portion of the bands in your data. The results of these measurements are then automatically recorded in a PeakFit-generated report, or they can be shown graphically.
[/toggle] [toggle border=’2′ title=’For Chromatography’] PeakFit includes 8 different built-in equations for asymmetric peaks typically found in chromatography data including the Exponentially-Modified Gaussian, the Haarhoff-Van der Linde, the NLC, the Giddings and even a Half-Gaussian Modified Gaussian, an experimental model similar to the EMG, but used to describe intra-column originated asymmetry. The peak area is computed directly as a parameter within each of these functions, ensuring accuracy and enabling computation of confidence limits. PeakFit reports column efficiency, resolution, first moment (center of mass), second moment, center (mode), as well as peak width at base and half maximum, and asymmetry at base and 10 percent of maximum. PeakFit can even deconvolve your chromatographic detector response, so you can analyze your data without instrument induced asymmetry. PeakFit also supports AIA data file input protocol.
[/toggle] [toggle border=’2′ title=’For Signal Component Analysis’] Even though PeakFit is widely used by chemists, biologists, etc., many Electrical Engineers have found PeakFit’s statistical nonlinear fitting techniques extremely useful for separating overlapping signals.
[/toggle] [toggle border=’2′ title=’A True Voigt Function’] In Spectroscopy, instrument and Doppler broadening effects create a Gaussian line shape, while natural and collision broadening cause a Lorentzian line shape. The Voigt function is a convolution of both the Gaussian and Lorentzian functions. Most analysis packages that offer a Voigt function use an approximation with very limited precision. PeakFit actually uses a closed-form solution to precisely calculate the function analytically. PeakFit has four different Voigt functions, so you can fit the parameters you’re most interested in, including the individual widths of both the Gaussian and Lorentzian components, and also the amplitude and area of the Voigt function. PeakFit’s precise calculation of the Voigt function is crucial to the accuracy of your analysis.
[/toggle] [toggle border=’2′ title=’Nonlinear Peak Separation and Analysis for Spectroscopy, Chromatography and Electrophoresis’] PeakFit separates and analyzes nonlinear peak data better, more accurately and more conveniently than your lab instrument – here’s why:
Don’t guess or rely on the accuracy of non-dedicated software made by hardware manufacturers. PeakFit far surpasses any other peak analysis package made!
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